Allogenic deproteinized bone combined with fibrin glue constructs tissue engineered bone in vivo

doi:10.3969/j.issn.2095-4344.2013.16.003

Abstract

Abstract:

BACKGROUND: Some experiments have demonstrated that allogenic deproteinized bone combined fibrin glue is more suitable for seed cells growth and proliferation in vitro.
OBJECTIVE: To observe the difference between allogenic deproteinized bone combined fibrin glue and allogenic deproteinized bone alone as scaffold materials for bone tissue engineering.
METHODS: Twenty-eight New Zealand white rabbits were randomly divided into three groups: allogenic deproteinized bone combined fibrin glue composited with mesenchymal stem cells was implanted in experiment group, allogenic deproteinized bone composited with mesenchymal stem cells implanted in control group, and allogenic deproteinized bone alone implanted in blank group.
RESULTS AND CONCLUSION: With the duration of implantation, the alkaline phosphatase activity of eachgroup was increased gradually. The experiment group was higher than control group and blank group in the alkaline phosphatase activity (P < 0.01). New bone tissue formed in both the experiment group and control group at 4 and 8 weeks after implantation, and there were more new bone tissues formed in the experiment group (P < 0.01). In the blank group, there was no new bone formed. Allogenic deproteinized bone combined fibrin glue is more suitable for mesenchymal stem cells than allogenic deproteinized bone alone, and it can be used as a scaffold for bone tissue engineering.

Key words: biomaterials, tissue-engineered bone materials, allogeneic, deproteinized bone, fibrin glue, bone marrow mesenchymal stem cells, bone tissue engineeringr

CLC Number:

R318

Miao Xu-dong, Pei Guo-xian, Yan Qiao-sheng, Jia Jing, Xu You. Allogenic deproteinized bone combined with fibrin glue constructs tissue engineered bone in vivo[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(16): 2869-2873.

Figures/Tables 5

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